CDKN2D Knockout cell line (U-251 MG)

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CDKN2D Knockout cell line (U-251 MG) Order

CDKN2D Knockout cell line (U-251 MG)
Catalog#
YKO-HT12750
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
CDKN2D Knockout cell line (U-251 MG)
Order

Detailed Product Information

Product category
KO Cell Lines
Cell name
CDKN2D Knockout cell line (U-251 MG)
Species
Human
Gene Name
CDKN2D
Gene ID
1032
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the CDKN2D gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the U-251 MG cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, CDKN2D gene knockout CDKN2D Knockout cell line (U-251 MG) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
CDKN2D Knockout cell line (U-251 MG)
CDKN2D Knockout cell line (U-251 MG)
Catalog#
YKO-HT12750
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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  • CDKN2D Knockout cell line (HeLa)

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